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Expression of microRNAs (133b and 138) and Correlation with Echocardiographic Parameters in Patients with Alcoholic Cardiomyopathy

Author(s):

Leiliandry de Araújo Melo, Maria Mariana Barros Melo da Silveira, Isabelle Cecília de Vasconcellos Piscoya, Victor Artur Eulálio Brasileiro, Isabela Cristina Cordeiro Farias, Kleyton Palmeira do Ó, Carlos Guilhermo Piscoya Roncal, Raul Emídio Lima, Dário Celestino Sobral Filho and Luydson Richardson Silva Vasconcelos*   Pages 1 - 9 ( 9 )

Abstract:


Introduction: Alcoholic cardiomyopathy (ACM) is a disease of difficult diagnosis. The real mechanisms related to its pathophysiology are not fully understood.

Objective: The aims of this study were to investigated whether miR-133b and miR-138 could be associated with ACM.

Method: Forty-four patients were included comprising 24 with ACM and 20 with cardiomyopathies of different etiologies (control group). Real time PCR was performed to verify the relative expression among the studied groups. In the statistical analysis, the quantitative variables t-student Mann-Whitney and correlation of Pearson tests were calculated, while the qualitative variable comprised the chi-square test, p<0.05 being considered statistically significant.

Results: There was no association between the clinical and sociodemographic characteristics between the groups. The patients with ACM presented downregulation of miR-133b in comparison with control patients (p=0.004). On the other hand, for the miR-138 there was no association when the ACM group was compared with the control group. The presence of miR-133b among cases and controls was not correlated with any of the echocardiographic parameters. However, the increase in the expression of miR-138 was correlated with an increase in the ejection fraction (r=0.28, p=0.01) and the diameter of the left atrium (r=0.23, p=0.04) in patients with ACM.

Conclusion: The downregulation of miR-133b might be a marker for ACM and, in addition miR-138 could be used to correlate the increase in ejection fraction with and normalization of the diameter of the left atrium diameter in patients with this disease.

Keywords:

Alcoholic cardiomyopath, microRNA, echocardiography, biomarkers, parameter diagnostimicroRNA, parameters, diagnostic

Affiliation:

Faculdade de Ciências Médicas/ Universidade de Pernambuco – FCM/UPE, Faculdade de Ciências Médicas/ Universidade de Pernambuco – FCM/UPE, Faculdade de Ciências Médicas/ Universidade de Pernambuco – FCM/UPE, Faculdade de Ciências Médicas/ Universidade de Pernambuco – FCM/UPE, Faculdade de Ciências Médicas/ Universidade de Pernambuco – FCM/UPE, Faculdade de Ciências Médicas/ Universidade de Pernambuco – FCM/UPE, Pronto Socorro Cardiológico de Pernambuco – PROCAPE, Instituto Aggeu Magalhães/ Fundação Oswaldo Cruz de Pernambuco – Fiocruz-PE, Faculdade de Ciências Médicas/ Universidade de Pernambuco – FCM/UPE, Faculdade de Ciências Médicas/ Universidade de Pernambuco – FCM/UPE



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