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Molecular Evaluation of MicroRNA-146 Gene Variability (rs2910164 C> G) and its Association with Increased Susceptibility to Coronary Artery Disease

[ Vol. 9 , Issue. 5 ]

Author(s):

Rashid Mir*, Imadeldin Elfaki, Chandan Jha, Jamsheed Javid, Suriya Rehman, Shaheena Banu, Mohammad Muzaffar Mir, Abdullatif Taha Babakr and Sukh Mohinder Singh Chahal   Pages 363 - 372 ( 10 )

Abstract:


Aim: Apart from the modifiable risk factors, genetic factors are believed to influence the outcome of Coronary Artery Diseases (CAD). Under the genetic factors, miRNA polymorphisms, namely Hsa-miR-146a-5p (rs2910164) have become an important tool to study the mechanism that underlies the pathogenesis of this disease. Therefore, we investigated the association of miR-146a gene variations with susceptibility of coronary artery diseases.

Methodology: This study was conducted on 100 CAD patients and 117 matched healthy individuals. Genotyping of the Hsa-miR-146a-5p C>G gene variation was performed by using Amplification Refractory Mutation System PCR method (ARMS-PCR).

Results: The distribution of Hsa-miR-146a-5p rs2910164 C>G genotypes observed between patients and controls was significantly different (P=0.048). Moreover, the frequency of G allele (fG) was found to be significantly higher among patients than in controls (0.36 vs. 0.25). Our findings showed that the Hsa-miR-146a-5p C>G variant was associated with an increased risk of CAD in codominant inheritance model CC vs. CG genotype (OR = 1.84, 95% CI, 1.02-3.31; p=0.040) and (OR = 3.18, 95% CI, 1.02-9.9; p=0.045) for CC vs. GG genotype in dominant inheritance model. Whereas the G allele significantly increased the risk of coronary artery disease (OR =1,81, 95% CI, 1.18-2.78; p=0.006) compared to C allele. Taken together, these results demonstrated that miR-146a/rs2910164 is associated with susceptibility to coronary artery disease, providing novel insights into the genetic etiology and underlying biology of coronary artery disease.

Conclusion: Our findings indicated that Hsa-miR-146a-5p rs2910164 GG genotype and G allele are associated with increased susceptibility to Coronary Artery Disease. A larger sample size can be the key to progress in establishing the genetic co-relation of miRNA gene polymorphisms and cardiovascular diseases.

Keywords:

Hsa-miR-146a-5p, microRNAs, Coronary Artery Disease (CAD), gene polymorphism, amplification refractory mutation system (ARMS-PCR), miRNA polymorphisms.

Affiliation:

Department of Medical Lab Technology, Faculty of Applied Medical Sciences, Prince Fahd Bin Sultan Research Chair, University of Tabuk, Tabuk, Department of Biochemistry, Faculty of Science, University of Tabuk, Tabuk, Department of Human Genetics Punjabi University, Punjab, Department of Medical Lab Technology, Faculty of Applied Medical Sciences, Prince Fahd Bin Sultan Research Chair, University of Tabuk, Tabuk, Institute of Research and Medical Consultation, Imam Abdulrahman Bin Faisal University, Dammam, Sri Jayadeva Institute of Cardiovascular Science & Research, Bangalore, Department of Basic Medical Sciences (Medical Biochemistry), College of Medicine, University of Bisha, Bisha, Department of Biochemistry, Umm Al-Qura University, Makkah, Department of Biochemistry, Faculty of Science, University of Tabuk, Tabuk

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