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Fragmentation and Matching of Human MicroRNA Sequences in 3’utr

[ Vol. 9 , Issue. 5 ]

Author(s):

Michael S. Parker, Ambikaipakan Balasubramaniam, Floyd R. Sallee and Steven L. Parker*   Pages 378 - 394 ( 17 )

Abstract:


Aims: Definition of sense and antisense microRNA matches in 3’utr.

Background: Matches of mature microRNAs (m-miRs) in human 3’utr could be traced to mutations producing fragments of original m-miR sequences without physical separation. (The m-miR matches in 5’utr and cds should be by far fewer, but could follow similar patterns).

Objective: To ascertain if the sense and antisense m-miR fragments in 3’utr occur at similar or different levels.

Methods: Frequency of sense and antisense m-miR matches in 3'utr was examined in the range of 7-22 nucleotides.

Results: The fragmentation occurs at gene level by mutation within one of the paired m-miRs, which upon transcription results in increased interactive capability for both former pre-micro (premir) RNA stem partners. The non-mutated stem partner can persist in 3’utr sequences, as is apparent from significant presence of miR-619-5p and miR-5096 and some conservation of 20 other simian- specific m-miR sequences. However, most of m-mir sequences in 3’utr are extensively fragmented, with low preservation of long matches. In flanks of individual m-miR embeds the mutated pre-mir positions are to a degree defined specifically.

Conclusion: The m-mir matches of various sizes in 3’utr apparently reflect accumulation, on a phylogenetic time scale, of in-sequence point mutations. Across human 3’utr this fragmentation is significantly less for evolutionarily recent human m-miRs that originate in simians compared to human m-miRs first appearing in lower primates, and especially to human m-miRs introduced in nonprimates.

Keywords:

microRNA phylogeny, simian microRNAs, microRNA mRNA segments, sense matching, antisense matching, miR-619-5p.

Affiliation:

Department of Microbiology and Molecular Cell Sciences, University of Memphis, Memphis TN 38152, Department of Surgery, University of Cincinnati School of Medicine, Cincinnati, OH 42567, Department of Psychiatry, University of Cincinnati School of Medicine, Cincinnati OH 42567, Department of Pharmacology, University of Tennessee Health Science Center, Memphis, TN 38163

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