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Assessment of MicroRNA-15a and MicroRNA-16-1 Salivary Level in Oral Squamous Cell Carcinoma Patients

[ Vol. 10 , Issue. 1 ]

Author(s):

Maryam Koopaie*, Soheila Manifar and Shahab Shokouhi Lahiji   Pages 74 - 79 ( 6 )

Abstract:


Background: Squamous Cell Carcinoma (SCC) includes more than 90% of malignancies of the oral cavity. Early diagnosis could effectively improve patients' quality of life and treatment outcomes of oral cancers. MicroRNAs as non-encoding genes have great potential to initiate or suppress cancer progression. Recent studies have shown that disruption of micro-RNA regulation is a common occurrence in cancers.

Objective: This study set out to evaluate the expression of microRNA-15a (miR-15a) and microRNA- 16-1 (miR-16-1) in the saliva of Oral Squamous Cell Carcinoma (OSCC) patients in comparison with a healthy control group.

Methods: This case-control study was performed on fifteen patients with OSCC and fifteen healthy volunteers as the control group. A 5 ml of non-stimulating whole saliva was collected by spitting method from patients and controls and stored at -70°C. The expression of miR-15a and miR-16-1 was investigated using quantitative Reverse-Transcription Polymerase Chain Reaction (RT-qPCR).

Results: MiR-15a and miR-16-1 were downregulated in OSCC patients compared with the control group (p<0.001). The sensitivity of miR-15a and miR-16-1 in differentiating OSCC patients from healthy individuals was 93.3% and 86.67%, respectively, and their specificity was 86.67% and 92.33%, respectively. The diagnostic accuracy of miR-15a was 90%, and miR-16-1 was 93.3%.

Conclusion: The present study showed a decrease in the relative expression of miR-15a and miR-16-1 in OSCC patients compared with healthy individuals. It is probable to introduce salivary values of miR-15a and miR-16-1 as a non-invasive tool for early detection of OSCC. Decreased expression of miR-15a and miR-16-1 in OSCC indicates the possible effective role of these genes in OSCC etiopathogenesis.

Keywords:

Squamous Cell Carcinoma (SCC), microRNA-15a (miR-15a), microRNA-16-1 (miR-16-1), saliva, RT-qPCR, OSCC.

Affiliation:

Department of Oral Medicine, School of Dentistry, Tehran University of Medical Sciences, Tehran, Department of Oral Medicine, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Department of Oral Medicine, School of Dentistry, Tehran University of Medical Sciences, Tehran



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